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  1. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
  2. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83
  3. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83, Issue 4, December 2013
  4. Direct Organogenesis in Punica granatum L. cv. Kandhari Kabuli from Hypocotyl Explants
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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83, Issue 4, December 2013
Bacterial Community Structure from the Perspective of the Uranium Ore Deposits of Domiasiat in India
Identification of Rice Blast Resistance Gene Pi9 from Indian Rice Land Races with STS Marker and Its Verification by Virulence Analysis
Postharvest Dry Storage Treatments Enhance Vase Life of Cut Scapes of Nerine sarniensis cv. Red
Urease Activity in Various Agro-ecological Sub-regions of Black Soil Regions of India
Mechanisms of Differential Manganese Uptake Efficiency in Winter Cereals at Generative Phase
Quantitative evaluation reveals taxonomic over-splitting in extinct marine invertebrates: implications in conserving biodiversity
Fipronil-Induced Biochemical Alterations During Oral Subacute Toxicity in Buffalo Calves
Effect of Sub-Acute Toxicity of Bifenthrin on Antioxidant status and Hematology After its Oral Exposure in Goats
Surface Plasmon Resonance Sensogram Based Characterization of Antibodies Raised Against Intact Teliospores and Purified Diagnostic Antigen for Development of Nano-Immunosensor for Fungal Spore Antigen of Karnal Bunt (Tilletia indica) of Wheat
Bacteria-induced systemic resistance and growth promotion in Glycine max L. Merrill upon challenge inoculation with Fusarium oxysporum
Direct Organogenesis in Punica granatum L. cv. Kandhari Kabuli from Hypocotyl Explants
Diversity and Density of Aquatic Insects in the Lower Reach of River Moirang, Manipur, North East India
Bleaching: The Driving Force of Scleractinian New Recruitment at Little Andaman Island, Andaman and Nicobar Islands
Primary Production in River Birupa, India
Distribution of Gastrin Immune Reactive Cells in the Alimentary Canal of a Stomachless Hill Stream Loach, Lepidocephalus guntea (Hamilton): An Immuno-Histochemical study
Cyto-histopathological Alterations in the Liver of Azadirachtin Treated Catfish, Heteropneustes fossilis
Development and Evaluation of PCR Assay Based on Outer Membrane Protein 22 Gene for Genus Specific Diagnosis of Brucella spp
Isolation and Characterization of Pasteurella multocida from Poultry and Deer
Neuronal Morphology and Spine Density of the Visual Wulst of the Strawberry Finch, Estrilda amandava
Responses of Castor Bean (Ricinus communis L.) to Lead Stress
Biochemical and Physiological Effect of Excess Manganese (Mn) in Bean (Phaseolus vulgaris L. cv. Strike)
Estimation of Resource Availability with Special Reference to Nonhuman Primates in a Rainforest Region in the Central Western Ghats, South India
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83, Issue 3, September 2013
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83, Issue 2, June 2013
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83, Issue 1, March 2013
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 82

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Direct Organogenesis in Punica granatum L. cv. Kandhari Kabuli from Hypocotyl Explants

Content Provider SpringerLink
Author Kanwar, Kamlesh Thakur, Ajay Kumar Parmar, Nehanjali
Copyright Year 2013
Abstract A high efficiency plant regeneration protocol has been developed for Punica granatum L. cv. Kandhari Kabuli using hypocotyl explants. Hypocotyl explants (about 0.5–1.0 cm in size) were procured from 15 to 20-day-old in vitro germinated seedlings. The highest frequency of shoot regeneration (68.21 %) along with 3.18 average number of shoots per explant was obtained via direct organogenesis from hypocotyl explants cultured on Murashige and Skoog’s medium supplemented with 2.0 mg/l 6-benzyl amino purine and 1.5 mg/l α-naphthalene acetic acid. High percentage root regeneration (85.62 %) in in vitro developed shoots was obtained on half strength Murashige and Skoog’s medium supplemented with 0.10 mg/l α-naphthalene acetic acid and 0.02 % activated charcoal. Pomegranate plantlets were able to regenerate within 6 weeks and well rooted plantlets were successfully acclimatized in plastic cups containing autoclaved peat moss. They were weaned in a growth room and finally moved to a greenhouse. This is the first report on direct organogenesis in P. granatum L. cv. Kandhari Kabuli using hypocotyl explants. This regeneration protocol will be very useful for undertaking genetic manipulation providing a value addition to pomegranate propagation in future.
Starting Page 569
Ending Page 574
Page Count 6
File Format PDF
ISSN 03698211
Journal Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
Volume Number 83
Issue Number 4
e-ISSN 22501746
Language English
Publisher Springer India
Publisher Date 2013-04-06
Publisher Place India
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Punica granatum L. Life Sciences Hardening Plant Biochemistry Shoot regeneration Nucleic Acid Chemistry Behavioural Sciences Hypocotyl explants Direct organogenesis
Content Type Text
Resource Type Article
Subject Environmental Science Agricultural and Biological Sciences
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