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  1. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
  2. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83
  3. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83, Issue 4, December 2013
  4. Development and Evaluation of PCR Assay Based on Outer Membrane Protein 22 Gene for Genus Specific Diagnosis of Brucella spp
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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83, Issue 4, December 2013
Bacterial Community Structure from the Perspective of the Uranium Ore Deposits of Domiasiat in India
Identification of Rice Blast Resistance Gene Pi9 from Indian Rice Land Races with STS Marker and Its Verification by Virulence Analysis
Postharvest Dry Storage Treatments Enhance Vase Life of Cut Scapes of Nerine sarniensis cv. Red
Urease Activity in Various Agro-ecological Sub-regions of Black Soil Regions of India
Mechanisms of Differential Manganese Uptake Efficiency in Winter Cereals at Generative Phase
Quantitative evaluation reveals taxonomic over-splitting in extinct marine invertebrates: implications in conserving biodiversity
Fipronil-Induced Biochemical Alterations During Oral Subacute Toxicity in Buffalo Calves
Effect of Sub-Acute Toxicity of Bifenthrin on Antioxidant status and Hematology After its Oral Exposure in Goats
Surface Plasmon Resonance Sensogram Based Characterization of Antibodies Raised Against Intact Teliospores and Purified Diagnostic Antigen for Development of Nano-Immunosensor for Fungal Spore Antigen of Karnal Bunt (Tilletia indica) of Wheat
Bacteria-induced systemic resistance and growth promotion in Glycine max L. Merrill upon challenge inoculation with Fusarium oxysporum
Direct Organogenesis in Punica granatum L. cv. Kandhari Kabuli from Hypocotyl Explants
Diversity and Density of Aquatic Insects in the Lower Reach of River Moirang, Manipur, North East India
Bleaching: The Driving Force of Scleractinian New Recruitment at Little Andaman Island, Andaman and Nicobar Islands
Primary Production in River Birupa, India
Distribution of Gastrin Immune Reactive Cells in the Alimentary Canal of a Stomachless Hill Stream Loach, Lepidocephalus guntea (Hamilton): An Immuno-Histochemical study
Cyto-histopathological Alterations in the Liver of Azadirachtin Treated Catfish, Heteropneustes fossilis
Development and Evaluation of PCR Assay Based on Outer Membrane Protein 22 Gene for Genus Specific Diagnosis of Brucella spp
Isolation and Characterization of Pasteurella multocida from Poultry and Deer
Neuronal Morphology and Spine Density of the Visual Wulst of the Strawberry Finch, Estrilda amandava
Responses of Castor Bean (Ricinus communis L.) to Lead Stress
Biochemical and Physiological Effect of Excess Manganese (Mn) in Bean (Phaseolus vulgaris L. cv. Strike)
Estimation of Resource Availability with Special Reference to Nonhuman Primates in a Rainforest Region in the Central Western Ghats, South India
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83, Issue 3, September 2013
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83, Issue 2, June 2013
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83, Issue 1, March 2013
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 82

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Cloning, Sequencing and Expression of Immunodominant Outer Membrane Protein Omp 22 from Brucella Spp.

Thesis

Development and Evaluation of PCR Assay Based on Outer Membrane Protein 22 Gene for Genus Specific Diagnosis of Brucella spp

Content Provider SpringerLink
Author Pawar, Hitesh N. Kaur, Daljit Minhas, Priyanka Deka, Dipak Agrawal, Ravi Kant
Copyright Year 2013
Abstract Brucellosis is a worldwide zoonosis and a significant cause of loss of health in human and animals. Conventionally, definitive diagnosis is done by isolation of brucellae which is time consuming, technically demanding and may be hazardous. The present study was aimed to develop PCR assay based on evolutionarily conserved outer membrane protein 22 gene for rapid diagnosis of Brucella spp. A set of primer was designed after multiple alignment of outer membrane protein 22 gene sequences by clustalW. PCR amplification resulted in single specific amplicon of 639 bp in all the Brucella strains tested. The primers were found to be highly sensitive and specific. PCR assay developed may be used for clinical diagnosis of Brucella infection.
Starting Page 615
Ending Page 619
Page Count 5
File Format PDF
ISSN 03698211
Journal Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
Volume Number 83
Issue Number 4
e-ISSN 22501746
Language English
Publisher Springer India
Publisher Date 2013-04-06
Publisher Place India
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Polymerase chain reaction Life Sciences Plant Biochemistry Nucleic Acid Chemistry Behavioural Sciences Brucella Outer membrane protein 22 Brucellosis Diagnosis
Content Type Text
Resource Type Article
Subject Environmental Science Agricultural and Biological Sciences
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