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  1. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
  2. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84
  3. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84, Issue 1, March 2014
  4. Use of Molecular and Protein Based Markers for Accessing Genetic Diversity Among Hippophae salicifolia D. Don Genotypes of Uttarakhand
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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84, Issue 4, December 2014
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84, Issue 3, September 2014
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84, Issue 2, June 2014
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84, Issue 1, March 2014
Cytology as an Important Tool for Solving Evolutionary Problems in Angiosperms
Crimean-Congo Hemorrhagic Fever: Current Scenario in India
Growth Behaviour of Transgenic Cotton with Peanut Intercropping System Using Modified Fertilization Technique
Assay of Nutritional Composition and Antioxidant Activity of Three Dioecious Momordica Species of South East Asia
Down-Regulation of Acid and Alkaline Phosphatases Induced After Prolonged Diclofenac Use in Experimental Mouse Bone
Use of Molecular and Protein Based Markers for Accessing Genetic Diversity Among Hippophae salicifolia D. Don Genotypes of Uttarakhand
Identification of RAPD Markers Associated with Morphological, Biochemical and Ionomic Characteristics in Indian Tomato Genotypes
Sunlight Irradiation Induced Green Synthesis of Stable Silver Nanoparticles Using Citrus limon Extract
Bryophyte Invasion on Famous Archaeological Site of Ahom Dynasty ‘Talatal Ghar’ of Sibsagar, Assam (India)
Natural Products Modulate Eicosanoid Mediated Nodulation in Poekilocerus pictus Fab. (Acrididae, Orthoptera)
Feed Utilization, Gonadal Maturation, Carcass Composition and Stress of the Catfish, Heteropneustes fossilis (Bloch) Fed with Animal Viscera-Based Diets at Varied Temperatures
Comparative Pattern of Reproductive Traits in Labeo rohita (Hamilton 1822) from Six Tropical Rivers of Ganges Basin: A New Insight
Phenotypic Variations Among Three Populations of Chirruh Snowtrout, Schizothorax esocinus (Heckel, 1838) in Kashmir Himalaya with Insights from Truss Network System
Haemato-biochemical Responses in Cyprinus carpio (Linnaeus, 1758) Fry Exposed to Sub-lethal Concentration of a Phenylpyrazole Insecticide, Fipronil
Pattern of Meristic and Morphometric Variations Between Wild and Captive Stocks of Endangered Tor putitora (Hamilton 1822) Using Multivariate Statistical Analysis Methods
Organization and Classification of Cytochrome P450 Genes in Castor (Ricinus communis L.)
Effect of Bacillus subtilis Strain CKT1 as Inoculum on Growth of Tomato Seedlings Under Net House Conditions
Plant Growth Promoting and Metal Bioadsorption Activity of Metal Tolerant Pseudomonas aeruginosa Isolate Characterized from Uranium Ore Deposit
Afr1p Expression During Mitosis is Dependent on Mpk1p in Saccharomyces cerevisiae
RAPD and Nuclear rDNA ITS Polymorphism Within Macrophomina phaseolina Isolated from Arid Legumes of Western Rajasthan
Role of 24-Epibrassinolide, Putrescine and Spermine in Salinity Stressed Adiantum capillus-veneris Leaves
A Liquid Culture System for Improved Micropropagation of Mature Acacia nilotica (L.) Del. ssp. indica and Ex Vitro Rooting
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 82

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Use of Molecular and Protein Based Markers for Accessing Genetic Diversity Among Hippophae salicifolia D. Don Genotypes of Uttarakhand

Content Provider SpringerLink
Author Gupta, Sanjay Mohan Gupta, Sanjay Singh, Sadhna Kumar, Anil Sharma, Netrapal
Copyright Year 2013
Abstract In order to study the genetic diversity among 16 genotypes of Hippophae salicifolia D. Don of Uttarakhand region, random amplified polymorphic DNA (RAPD)–PCR analysis along with protein profiling of seed storage proteins was carried out for selection of superior genotype having high economic importance. A total of 21 RAPD primers were assayed for their specificity in detecting genetic variability in H. salicifolia genotypes, of which 11 RAPD primers were highly reproducible and were found suitable for use in PCR analysis. A total of 198 bands were scored corresponding to an average of 10.8 bands per primer with 138 bands showing polymorphism (73.2 %) with similarity coefficient ranging from 0.011 to 0.999. A dendrogram constructed based on the UPGMA clustering method revealed two major clusters. Cluster-I comprises of 13 cultivars, while cluster-II includes the remaining three cultivars. The cultivar ST-4, ST-5 and ST-16 were quite unique from the remaining cultivars as evident from the dendrogram. The analysis of total seed storage protein (quantitative) and protein profiling (qualitative) among 16 genotypes of H. salicifolia was performed to indentify novel proteins of important functional attributes. SDS-PAGE based generated profiles of seed proteins showed major differences in banding patterns among these genotypes with respect to altitude besides differences in seed protein contents. This study helps in the selection of superior genotype of H. salicifolia having higher economic importance by using developed molecular and protein based markers in Uttarakhand regions.
Starting Page 47
Ending Page 54
Page Count 8
File Format PDF
ISSN 03698211
Journal Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
Volume Number 84
Issue Number 1
e-ISSN 22501746
Language English
Publisher Springer India
Publisher Date 2013-06-20
Publisher Place India
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Life Sciences SDS-PAGE Plant Biochemistry Nucleic Acid Chemistry Elaeagnaceae RAPD Behavioural Sciences Genetic diversity Seabuckthorn Hippophae salicifolia genotypes
Content Type Text
Resource Type Article
Subject Environmental Science Agricultural and Biological Sciences
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