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  1. Journal of the American Oil Chemists' Society
  2. Journal of the American Oil Chemists' Society : Volume 80
  3. Journal of the American Oil Chemists' Society : Volume 80, Issue 3, March 2003
  4. Enzymatic conversion of steryl esters to free sterols
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Journal of the American Oil Chemists' Society : Volume 94
Journal of the American Oil Chemists' Society : Volume 93
Journal of the American Oil Chemists' Society : Volume 92
Journal of the American Oil Chemists' Society : Volume 91
Journal of the American Oil Chemists' Society : Volume 90
Journal of the American Oil Chemists' Society : Volume 89
Journal of the American Oil Chemists' Society : Volume 88
Journal of the American Oil Chemists' Society : Volume 87
Journal of the American Oil Chemists' Society : Volume 86
Journal of the American Oil Chemists' Society : Volume 85
Journal of the American Oil Chemists' Society : Volume 84
Journal of the American Oil Chemists' Society : Volume 83
Journal of the American Oil Chemists' Society : Volume 82
Journal of the American Oil Chemists' Society : Volume 81
Journal of the American Oil Chemists' Society : Volume 80
Journal of the American Oil Chemists' Society : Volume 80, Issue 12, December 2003
Journal of the American Oil Chemists' Society : Volume 80, Issue 11, November 2003
Journal of the American Oil Chemists' Society : Volume 80, Issue 10, October 2003
Journal of the American Oil Chemists' Society : Volume 80, Issue 9, September 2003
Journal of the American Oil Chemists' Society : Volume 80, Issue 8, August 2003
Journal of the American Oil Chemists' Society : Volume 80, Issue 7, July 2003
Journal of the American Oil Chemists' Society : Volume 80, Issue 6, June 2003
Journal of the American Oil Chemists' Society : Volume 80, Issue 5, May 2003
Journal of the American Oil Chemists' Society : Volume 80, Issue 4, April 2003
Journal of the American Oil Chemists' Society : Volume 80, Issue 3, March 2003
Structural characterization of triacylglycerols using electrospray ionization-MS$^{n}$ ion-trap MS
Detection of olive oil adulteration using principal component analysis applied on total and regio FA content
Two-stage solvent extraction of seeds of Hibiscus abelmoschus linn: Lipid and FA compositions
Monitoring milk fat fractionation: Filtration properties and crystallization kinetics
TAG composition of ewe's milk fat. Detection of foreign fats
Identification and quantification of neuroactive N-acylethanolamines in cottonseed processing fractions
FA selectivity of lipases in acyl-transfer reactions with acetate esters of polyols in organic media
Partial purification of nigella sativa L. Seed lipase and its application in hydrolytic reactions. Enrichment of γ-linolenic acid from borage oil
Enzymatic conversion of steryl esters to free sterols
Effect of fruit stoning on olive oil quality
Chromatic evolution of virgin olive oils submitted to an accelerated oxidation test
Physical analyses of gel-like behavior of binary mixtures of high- and low-melting fats
Lipid composition in tomato skin supercritical fluid extracts with high lycopene content
Frying quality and stability of low-and ultra-low-linolenic acid soybean oils
Preparation of alkenyl succinic anhydrides from vegetable oil FAME
Oil-free protein isolates from full-fat, dehulled mustard flour by microfiltration
Continuous abrasive method for mechanically fractionating flaxseed
Journal of the American Oil Chemists' Society : Volume 80, Issue 2, February 2003
Journal of the American Oil Chemists' Society : Volume 80, Issue 1, January 2003
Journal of the American Oil Chemists' Society : Volume 79
Journal of the American Oil Chemists' Society : Volume 78
Journal of the American Oil Chemists' Society : Volume 77
Journal of the American Oil Chemists' Society : Volume 76
Journal of the American Oil Chemists' Society : Volume 75
Journal of the American Oil Chemists' Society : Volume 74

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Enzymatic conversion of steryl esters to free sterols

Content Provider SpringerLink
Author Shimada, Yuji Nagao, Toshihiro Watanabe, Youichi Takagi, Yoshiaki Sugihara, Akio
Copyright Year 2003
Abstract Conversion of oleic acid phytosteryl esters (OASE) to free phytosterols (referred to as sterols) by an enzymatic process was attempted. Enzymatic hydrolysis of OASE reached a steady state at 55–60% hydrolysis, but addition of methanol (MeOH) significantly accelerated the conversion of OASE to sterols. Screening of commercially available enzymes indicated that Pseudomonas aeruginosa lipase was most effective for the conversion. Based on the study of several factors affecting the reaction, the optimal conditions were determined as follows: ratio of OASE to MeOH, 1∶2 (mol/mol); water content, 10 wt%; lipase amount, 20 U/g by weight of reaction mixture; temperature, 30°C. When the reaction was conducted for 48 h with stirring, the conversion reached 98%. FAME accumulated in the reaction mixture, but FFA did not, indicating that the FAME was poorly recognized as a substrate in the reverse conversion of sterols to OASE but the FFA was easily recognized as a substrate. The high conversion of OASE to sterols was therefore due to elimination of FFA from the reaction system. After the enzymatic reaction, the oil layer was fractionated at −20°C with 5 vol parts of n-hexane. Sterols were efficiently purified in the resulting precipitate (92% recovery, 99% purity).
Starting Page 243
Ending Page 247
Page Count 5
File Format PDF
ISSN 0003021X
Journal Journal of the American Oil Chemists' Society
Volume Number 80
Issue Number 3
e-ISSN 15589331
Language English
Publisher Springer-Verlag
Publisher Date 2003-01-01
Publisher Place Berlin, Heidelberg
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Enzymatic conversion lipase Pseudomonas aeruginosa solvent fractionation sterol steryl ester Chemistry/Food Science Analytical Chemistry Biotechnology Industrial Chemistry/Chemical Engineering Biomaterials Agriculture
Content Type Text
Resource Type Article
Subject Organic Chemistry Chemical Engineering
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