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  1. Journal of the American Oil Chemists' Society
  2. Journal of the American Oil Chemists' Society : Volume 86
  3. Journal of the American Oil Chemists' Society : Volume 86, Issue 7, July 2009
  4. Characterization of Cross-Linked Lipase Aggregates
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Journal of the American Oil Chemists' Society : Volume 94
Journal of the American Oil Chemists' Society : Volume 93
Journal of the American Oil Chemists' Society : Volume 92
Journal of the American Oil Chemists' Society : Volume 91
Journal of the American Oil Chemists' Society : Volume 90
Journal of the American Oil Chemists' Society : Volume 89
Journal of the American Oil Chemists' Society : Volume 88
Journal of the American Oil Chemists' Society : Volume 87
Journal of the American Oil Chemists' Society : Volume 86
Journal of the American Oil Chemists' Society : Volume 86, Issue 12, December 2009
Journal of the American Oil Chemists' Society : Volume 86, Issue 11, November 2009
Journal of the American Oil Chemists' Society : Volume 86, Issue 10, October 2009
Journal of the American Oil Chemists' Society : Volume 86, Issue 9, September 2009
Journal of the American Oil Chemists' Society : Volume 86, Issue 8, August 2009
Journal of the American Oil Chemists' Society : Volume 86, Issue 7, July 2009
Purification of GLA-Triglycerides from Evening Primrose Oil by Gravimetric Column Chromatography
Characterization of Oil Extracted from Buriti Fruit (Mauritia flexuosa) Grown in the Brazilian Amazon Region
Profiling of Phytosterols, Tocopherols and Tocotrienols in Selected Seed Oils from Botswana by GC–MS and HPLC
Color Measurements in Blue-Tinted Cups for Virgin-Olive-Oil Tasting
Characterization of Cross-Linked Lipase Aggregates
Milk Thistle Seed Oil Constituents from Different Varieties Grown in Iran
Products Formed During Thermo-oxidative Degradation of Phytosterols
Characterization of Turkish Virgin Olive Oils Produced from Early Harvest Olives
Synthesis of Sodium (+)-(12S,13R)-Epoxy-cis-9-octadecenyl Sulfonate from Vernonia Oil
Chemical and Enzymatic Interesterification of a Blend of Palm Stearin: Soybean Oil for Low trans-Margarine Formulation
Comparative Oxidative Stability of Fatty Acid Alkyl Esters by Accelerated Methods
Plant Sterol and Policosanol Characterization of Hexane Extracts from Grain Sorghum, Corn and their DDGS
Journal of the American Oil Chemists' Society : Volume 86, Issue 6, June 2009
Journal of the American Oil Chemists' Society : Volume 86, Issue 5, May 2009
Journal of the American Oil Chemists' Society : Volume 86, Issue 4, April 2009
Journal of the American Oil Chemists' Society : Volume 86, Issue 3, March 2009
Journal of the American Oil Chemists' Society : Volume 86, Issue 2, February 2009
Journal of the American Oil Chemists' Society : Volume 86, Issue 1, January 2009
Journal of the American Oil Chemists' Society : Volume 85
Journal of the American Oil Chemists' Society : Volume 84
Journal of the American Oil Chemists' Society : Volume 83
Journal of the American Oil Chemists' Society : Volume 82
Journal of the American Oil Chemists' Society : Volume 81
Journal of the American Oil Chemists' Society : Volume 80
Journal of the American Oil Chemists' Society : Volume 79
Journal of the American Oil Chemists' Society : Volume 78
Journal of the American Oil Chemists' Society : Volume 77
Journal of the American Oil Chemists' Society : Volume 76
Journal of the American Oil Chemists' Society : Volume 75
Journal of the American Oil Chemists' Society : Volume 74

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Characterization of Cross-Linked Lipase Aggregates

Content Provider SpringerLink
Author Prabhavathi Devi, B. L. A. Guo, Zheng Xu, Xuebing
Copyright Year 2009
Abstract Commercially available microbial lipases from different sources were immobilized as cross-linked enzyme aggregates (CLEAs) using different precipitants and glutaraldehyde as cross-linkers. These CLEAs were assayed based on esterification between lauric acid and n-propanol in solvent-free systems. Precipitants were found to have a profound influence on both specific activities and total activity recovery of CLEAs, as exemplified by Candida antarctica lipase B (CALB). Among the CLEAs of CALB studied, those obtained using PEG600, ammonium sulfate, PEG200 and acetone as precipitants were observed to attain over 200% total activity recovery in comparison with acetone powder directly precipitated from the liquid solution by acetone. PEG200 precipitated CLEA gave the best specific activity (139% relative to acetone powder). The results of kinetic studies showed that V $_{max}$/K $_{m}$ does not significantly change upon CLEA formation. This work presents a characterization of CLEAs based on an esterification activity assay, which is useful for exploring the synthetic application potential of CLEA technology with favorable perspectives.
Starting Page 637
Ending Page 642
Page Count 6
File Format PDF
ISSN 0003021X
Journal Journal of the American Oil Chemists' Society
Volume Number 86
Issue Number 7
e-ISSN 15589331
Language English
Publisher Springer-Verlag
Publisher Date 2009-05-27
Publisher Place Berlin, Heidelberg
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Lipase Cross-linked enzyme aggregates (CLEAs) Candida antarctica lipase B (CALB) Esterification activity Immobilization Biotechnology Food Science Agriculture Biomaterials Industrial Chemistry/Chemical Engineering
Content Type Text
Resource Type Article
Subject Organic Chemistry Chemical Engineering
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