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  1. Development Genes and Evolution
  2. Development Genes and Evolution : Volume 218
  3. Development Genes and Evolution : Volume 218, Issue 9, September 2008
  4. Larval RNAi in Drosophila?
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Development Genes and Evolution : Volume 227
Development Genes and Evolution : Volume 226
Development Genes and Evolution : Volume 225
Development Genes and Evolution : Volume 224
Development Genes and Evolution : Volume 223
Development Genes and Evolution : Volume 222
Development Genes and Evolution : Volume 221
Development Genes and Evolution : Volume 220
Development Genes and Evolution : Volume 219
Development Genes and Evolution : Volume 218
Development Genes and Evolution : Volume 218, Issue 11-12, December 2008
Development Genes and Evolution : Volume 218, Issue 10, October 2008
Development Genes and Evolution : Volume 218, Issue 9, September 2008
Vasa and nanos are coexpressed in somatic and germ line tissue from early embryonic cleavage stages through adulthood in the polychaete Capitella sp. I
The role of the segmentation gene hairy in Tribolium
NK homeobox genes with choanocyte-specific expression in homoscleromorph sponges
Identification of stage-specific larval camouflage associated genes in the swallowtail butterfly, Papilio xuthus
Larval RNAi in Drosophila?
Development Genes and Evolution : Volume 218, Issue 8, August 2008
Development Genes and Evolution : Volume 218, Issue 7, July 2008
Development Genes and Evolution : Volume 218, Issue 6, June 2008
Development Genes and Evolution : Volume 218, Issue 5, May 2008
Development Genes and Evolution : Volume 218, Issue 3-4, April 2008
Development Genes and Evolution : Volume 218, Issue 2, February 2008
Development Genes and Evolution : Volume 218, Issue 1, January 2008
Development Genes and Evolution : Volume 217
Development Genes and Evolution : Volume 216
Development Genes and Evolution : Volume 215
Development Genes and Evolution : Volume 214
Development Genes and Evolution : Volume 213
Development Genes and Evolution : Volume 212
Development Genes and Evolution : Volume 211
Development Genes and Evolution : Volume 210
Development Genes and Evolution : Volume 209
Development Genes and Evolution : Volume 208
Development Genes and Evolution : Volume 207
Development Genes and Evolution : Volume 206

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Larval RNAi in Drosophila?

Content Provider SpringerLink
Author Miller, Sherry C. Brown, Susan J. Tomoyasu, Yoshiri
Copyright Year 2008
Abstract RNA interference (RNAi) has become a common method of gene knockdown in many model systems. To trigger an RNAi response, double-stranded RNA (dsRNA) must enter the cell. In some organisms such as Caenorhabditis elegans, cells can take up dsRNA from the extracellular environment via a cellular uptake mechanism termed systemic RNAi. However, in the fruit fly Drosophila melanogaster, it is widely believed that cells are unable to take up dsRNA, although there is little published data to support this claim. In this study, we set out to determine whether this perception has a factual basis. We took advantage of traditional Gal4/upstream activation sequence (UAS) transgenic flies as well as the mosaic analysis with a repressible cell marker (MARCM) system to show that extracellular injection of dsRNA into Drosophila larvae cannot trigger RNAi in most Drosophila tissues (with the exception of hemocytes). Our results show that this is not due to a lack of RNAi machinery in these tissues as overexpression of dsRNA inside the cells using hairpin RNAs efficiently induces an RNAi response in the same tissues. These results suggest that, while most Drosophila tissues indeed lack the ability to uptake dsRNA from the surrounding environment, hemocytes can initiate RNAi in response to extracellular dsRNA. We also examined another insect, the red flour beetle Tribolium castaneum, which has been shown to exhibit a robust systemic RNAi response. We show that virtually all Tribolium tissues can respond to extracellular dsRNA, which is strikingly different from the situation in Drosophila. Our data provide specific information about the tissues amenable to RNAi in two different insects, which may help us understand the molecular basis of systemic RNAi.
Starting Page 505
Ending Page 510
Page Count 6
File Format PDF
ISSN 0949944X
Journal Development Genes and Evolution
Volume Number 218
Issue Number 9
e-ISSN 1432041X
Language English
Publisher Springer-Verlag
Publisher Date 2008-07-29
Publisher Place Berlin, Heidelberg
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword RNAi Systemic Tribolium castaneum (red flour beetle) Drosophila melanogaster (fruit fly) Injection Animal Genetics and Genomics Biochemistry Cell Biology Neurosciences Developmental Biology
Content Type Text
Resource Type Article
Subject Genetics Developmental Biology
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