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  1. Analytical and Bioanalytical Chemistry
  2. Analytical and Bioanalytical Chemistry : Volume 408
  3. Analytical and Bioanalytical Chemistry : Volume 408, Issue 17, July 2016
  4. Post-SELEX optimization of aptamers
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Analytical and Bioanalytical Chemistry : Volume 409
Analytical and Bioanalytical Chemistry : Volume 408
Analytical and Bioanalytical Chemistry : Volume 408, Issue 30, December 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 29, November 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 28, November 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 27, November 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 26, October 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 25, October 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 24, September 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 23, September 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 22, September 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 21, August 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 19, July 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 18, July 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 17, July 2016
Hollandaise challenge
Solution to Mohr’s method challenge
Peter Kapusta, Michael Wahl, and Rainer Erdmann (Eds.): Advanced photon counting: applications, methods, instrumentation
Recent developments in Förster resonance energy transfer (FRET) diagnostics using quantum dots
Gold nanoparticles assisted laser desorption/ionization mass spectrometry and applications: from simple molecules to intact cells
Microfabricated, amperometric, enzyme-based biosensors for in vivo applications
Disc-based microarrays: principles and analytical applications
Raman-based microarray readout: a review
Determination of elemental impurities in pharmaceutical products and related matrices by ICP-based methods: a review
Post-SELEX optimization of aptamers
DNA enrichment approaches to identify unauthorized genetically modified organisms (GMOs)
Application of whole genome shotgun sequencing for detection and characterization of genetically modified organisms and derived products
Advances in DNA metabarcoding for food and wildlife forensic species identification
The biochemical origins of the surface-enhanced Raman spectra of bacteria: a metabolomics profiling by SERS
Concurrent supercritical fluid chromatographic analysis of terpene lactones and ginkgolic acids in Ginkgo biloba extracts and dietary supplements
A GC/MS method for determination of succinylacetone in Arabidopsis thaliana
Identification and absolute quantification of enzymes in laundry detergents by liquid chromatography tandem mass spectrometry
1H NMR-based metabolite profiling workflow to reduce inter-sample chemical shift variations in urine samples for improved biomarker discovery
Development of a lateral flow immunoassay for rapid field detection of the red imported fire ant, Solenopsis invicta (Hymenoptera: Formicidae)
Carbon dots-decorated multiwalled carbon nanotubes nanocomposites as a high-performance electrochemical sensor for detection of H2O2 in living cells
An aptamer assay using rolling circle amplification coupled with thrombin catalysis for protein detection
Setup and validation of a convenient sampling procedure to promptly and effectively stabilize vitamin C in blood and plasma specimens stored at routine temperatures
Sequential SERS determination of aspirin and vitamin C using in situ laser-induced photochemical silver substrate synthesis in a moving flow cell
Optimization and comparison of bottom-up proteomic sample preparation for early-stage Xenopus laevis embryos
Multi-platform metabolomics assays for human lung lavage fluids in an air pollution exposure study
N-Glycan profile analysis of transferrin using a microfluidic compact disc and MALDI-MS
Application of a NMR-based untargeted quantitative metabonomic approach to screen for illicit salbutamol administration in cattle
Immobilized rolling circle amplification on extended-gate field-effect transistors with integrated readout circuits for early detection of platelet-derived growth factor
HPLC-UV method for evaluation of inhibitors of plasma amine oxidase using derivatization of an aliphatic aldehyde product with TRIS
Effects of dissolved organic matter (DOM) sources and nature of solid extraction sorbent on recoverable DOM composition: Implication into potential lability of different compound groups
Erratum to: LC-MS/MS determination of potential endocrine disruptors of cortico signalling in rivers and wastewaters
Analytical and Bioanalytical Chemistry : Volume 408, Issue 16, June 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 15, June 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 13, May 2016
Analytical and Bioanalytical Chemistry : Volume 397
Analytical and Bioanalytical Chemistry : Volume 391
Analytical and Bioanalytical Chemistry : Volume 380
Analytical and Bioanalytical Chemistry : Volume 379
Analytical and Bioanalytical Chemistry : Volume 378
Analytical and Bioanalytical Chemistry : Volume 373

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Book Review

Post-SELEX optimization of aptamers

Content Provider SpringerLink
Author Gao, Shunxiang Zheng, Xin Jiao, Binghua Wang, Lianghua
Copyright Year 2016
Abstract Aptamers are functional single-stranded DNA or RNA oligonucleotides, selected in vitro by SELEX (Systematic Evolution of Ligands by Exponential Enrichment), which can fold into stable unique three-dimensional structures that bind their target ligands with high affinity and specificity. Although aptamers show a number of favorable advantages such as better stability and easier modification when compared with the properties of antibodies, only a handful of aptamers have entered clinical trials and only one, pegaptanib, has received US Food and Drug Administration approval for clinical use. The main reasons that limit the practical application of aptamers are insufficient nuclease stability, bioavailability, thermal stability, or even affinity. Some aptamers obtained from modified libraries show better properties; however, polymerase amplification of nucleic acids containing non-natural bases is currently a primary drawback of the SELEX process. This review focuses on several post-SELEX optimization strategies of aptamers identified in recent years. We describe four common methods in detail: truncation, chemical modification, bivalent or multivalent aptamer construction, and mutagenesis. We believe that these optimization strategies should improve one or more specific properties of aptamers, and the type of feature(s) selected for improvement will be dependent on the application purpose.
Starting Page 4567
Ending Page 4573
Page Count 7
File Format PDF
ISSN 16182642
Journal Analytical and Bioanalytical Chemistry
Volume Number 408
Issue Number 17
e-ISSN 16182650
Language English
Publisher Springer Berlin Heidelberg
Publisher Date 2016-05-12
Publisher Place Berlin, Heidelberg
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Aptamer Optimization Truncation Chemical modification Polyvalent ligand Mutation Analytical Chemistry Biochemistry Laboratory Medicine Characterization and Evaluation of Materials Food Science Environmental Monitoring/Analysis
Content Type Text
Resource Type Article
Subject Analytical Chemistry Biochemistry
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