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  1. Analytical and Bioanalytical Chemistry
  2. Analytical and Bioanalytical Chemistry : Volume 408
  3. Analytical and Bioanalytical Chemistry : Volume 408, Issue 15, June 2016
  4. CIEF-CZE-MS applying a mechanical valve
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Analytical and Bioanalytical Chemistry : Volume 409
Analytical and Bioanalytical Chemistry : Volume 408
Analytical and Bioanalytical Chemistry : Volume 408, Issue 30, December 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 29, November 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 28, November 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 27, November 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 26, October 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 25, October 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 24, September 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 23, September 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 22, September 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 21, August 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 19, July 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 18, July 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 17, July 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 16, June 2016
Analytical and Bioanalytical Chemistry : Volume 408, Issue 15, June 2016
Robert Kellner Lecture awarded to ABC author Bernhard Lendl
Michael L. Johns, Einar O. Fridjonsson, Sarah Vogt, and Agnes Haber (Eds.):Mobile NMR and MRI. Developments and Applications
EC-QCL mid-IR transmission spectroscopy for monitoring dynamic changes of protein secondary structure in aqueous solution on the example of β-aggregation in alcohol-denaturated α-chymotrypsin
Label-free C-reactive protein electronic detection with an electrolyte-gated organic field-effect transistor-based immunosensor
Comparative studies of peak intensities and chromatographic separation of proteolytic digests, PTMs, and intact proteins obtained by nanoLC-ESI MS analysis at room and elevated temperatures
Mitigation of microtiter plate positioning effects using a block randomization scheme
Development and validation of an LC-ESI-MS/MS method for the simultaneous quantification of naproxen and sumatriptan in human plasma: application to a pharmacokinetic study
A Raman “spectroscopic clock” for bloodstain age determination: the first week after deposition
Determining the core, corona, and total size of CdSeS/ZnS quantum dots by SEC/QELS and TEM
Polydopamine-coated magnetic nanoparticles for isolation and enrichment of estrogenic compounds from surface water samples followed by liquid chromatography-tandem mass spectrometry determination
Stability issues in the determination of 19 urinary (free and conjugated) monohydroxy polycyclic aromatic hydrocarbons
Single particle analysis of herpes simplex virus: comparing the dimensions of one and the same virions via atomic force and scanning electron microscopy
A modified QuEChERS approach for the screening of dioxins and furans in sediments
CIEF-CZE-MS applying a mechanical valve
Ultra-trace levels analysis of microcystins and nodularin in surface water by on-line solid-phase extraction with high-performance liquid chromatography tandem mass spectrometry
Ageing of resin from Pinus species assessed by infrared spectroscopy
Raman imaging in geomicrobiology: endolithic phototrophic microorganisms in gypsum from the extreme sun irradiation area in the Atacama Desert
Detection of nitroaromatic explosives by new D–π–A sensing fluorophores on the basis of the pyrimidine scaffold
Comparison of piracetam measured with HPLC-DAD, HPLC-ESI-MS, DIP-APCI-MS, and a newly developed and optimized DIP-ESI-MS
Detection of protein adduction derived from dauricine by alkaline permethylation
Fluorescent single-stranded DNA-based assay for detecting unchelated Gadolinium(III) ions in aqueous solution
Streptavidin conjugation and quantification—a method evaluation for nanoparticles
A sandwich dipstick assay for ATP detection based on split aptamer fragments
A novel automatable enzyme-coupled colorimetric assay for endo-1,4-β-glucanase (cellulase)
Rapid screening of waterborne pathogens using phage-mediated separation coupled with real-time PCR detection
Development of a multi-class steroid hormone screening method using Liquid Chromatography/Tandem Mass Spectrometry (LC-MS/MS)
Analytical and Bioanalytical Chemistry : Volume 408, Issue 13, May 2016
Analytical and Bioanalytical Chemistry : Volume 397
Analytical and Bioanalytical Chemistry : Volume 391
Analytical and Bioanalytical Chemistry : Volume 380
Analytical and Bioanalytical Chemistry : Volume 379
Analytical and Bioanalytical Chemistry : Volume 378
Analytical and Bioanalytical Chemistry : Volume 373

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CIEF-CZE-MS applying a mechanical valve

Content Provider SpringerLink
Author Hühner, Jens Neusüß, Christian
Copyright Year 2016
Abstract Separation and determination of proteins by capillary isoelectric focusing (CIEF) and mass spectrometry (MS) are essential and complementary techniques in the field of bioanalysis. The hyphenation of these two techniques is challenging due to the nonvolatile substances required for the CIEF separation. An additional separation step prior to MS enables the removal of the nonvolatile substances. However, it is complicated due to the small transfer volume and the required high voltages in the CIEF process. In order to remove nonvolatile substances and transfer the analytes toward the mass spectrometer, we applied a four-port valve to couple CIEF online to capillary electrophoresis-mass spectrometry. To demonstrate the power of this concept, hemoglobin and glycated hemoglobin with an isoelectric point difference of 0.037 were separated via isoelectric focusing and characterized by MS. In general, this setup guaranties interference-free mass spectra and will provide an information-rich and sensitive top down protein characterization. Graphical abstract Interference free coupling of capillary isoelectric focusing to mass spectrometry by applying a mechanical valve. The focused proteins were tranferred from the isoelectric focusing to capillary electrophoresis by a mechanical valve. Afterwards, the transferred protein was sepearated from ionization interfering substances in the capillary electrophoresis prior to the mass spectrometry detection.
Starting Page 4055
Ending Page 4061
Page Count 7
File Format PDF
ISSN 16182642
Journal Analytical and Bioanalytical Chemistry
Volume Number 408
Issue Number 15
e-ISSN 16182650
Language English
Publisher Springer Berlin Heidelberg
Publisher Date 2016-04-06
Publisher Place Berlin, Heidelberg
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Capillary isoelectric focusing Mass spectrometry Online hyphenation Protein Two-dimensional separation Analytical Chemistry Biochemistry Laboratory Medicine Characterization and Evaluation of Materials Food Science Environmental Monitoring/Analysis
Content Type Text
Resource Type Article
Subject Analytical Chemistry Biochemistry
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